Nov 19, 2019 · We elaborate on how RNA 2′-OH acylation is being exploited to develop selective chemical probes that enable interrogation of RNA structure and function, and describe new developments and...

Jan 18, 2024 · We outline progress made in probing RNA structure and interactomes, controlling RNA function, RNA imaging, and analyzing RNA-small molecule interactions, all achieved in living cells through this simple chemical handle on the biopolymer.

Feb 16, 2014 · The 5' and 3' mean "five prime" and "three prime", which indicate the carbon numbers in the DNA's sugar backbone. The 5' carbon has a phosphate group attached to it and the 3' carbon a hydroxyl (-OH) group.

Mar 16, 2011 · Discover the key role of the 2’ hydroxyl in RNA, not found in DNA. Understand this vital structural difference between RNA and DNA.

Splicing of precursor tRNA transcripts (pre-tRNAs) is accomplished by specialized endonucleases that excise introns and ligases that join the remaining exon halves. RTCB functions as a tRNA ligase (Popow et al., 2011).

Oct 29, 1999 · These RTs recognize specific RNA structures in their individual primer-template RNAs to initiate cDNA synthesis from the 2′-OH group of a specific internal G residue (branching G residue). The resulting products (msDNA) consist of RNA and single-stranded DNA, sharing hardly any sequence homology.

We elaborate on how RNA 2′-OH acylation is being exploited to develop selective chemical probes that enable interrogation of RNA structure and function, and describe new developments and applications in the field.

Protecting groups which are stable in the presence of acid and base are clearly prime candidates for use in 2' protection. For example, the 2-nitrobenzyl group 2 can be removed photochemically and the 3,4- dimethoxybenzyl 3 group can be oxidatively cleaved.

In this review, we detail the structure and mechanism of over a dozen factors that govern Pol II initiation (e.g., TFIID, TFIIH, and Mediator), pausing, and elongation (e.g., DSIF, NELF, PAF, and P-TEFb).

PRIME is a P rotein- R NA I nteraction M od E lling program ,which includes TMalign (protein structural alignment) and SARA (RNA structural alignment) for searching template in the library. Protein and RNA structure coordinates are needed. The output is the protein RNA-complex structure model.