HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent. It is one of the twenty Good's buffers.

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is a HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. A Good's buffer substance, pKa = 7.55 at 20 ℃. It is a HEPES and an organosulfonic acid.

Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator.

HEPES, also known as 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer. It is effective in the pH 6.8-8.2 range, with a pKa of 7.48 at 25°C.

HEPES（4-羟乙基哌嗪乙磺酸） 是一种常用的 两性离子缓冲液，在37°C下的pKa为7.3。 在培养基中我们除了 碳酸氢钠 外，还应使用HEPES，因为重要的是要在培养基中保持足够的碳酸氢盐以达到营养目的。 如果需要，HEPES可以以10mM至25mM的最终浓度添加到 细胞培养基 中，以提供额外的缓冲能力。 较低的浓度通常不足以控制pH的波动，而较高的浓度通常是有毒的。 由 …

HEPES is commonly used as a biological buffering agent in biochemical research that helps maintain a stable pH in aqueous solutions. It is effective as a buffer, especially in the pH range of 6.8 to 8.2.

HEPES (4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid) is a commonly used cell culture buffer that can stabilize the pH value of the cell culture medium, provide a suitable ionic environment, protect cells from external environmental …

Mar 6, 2025 · HEPES (CAS 7365-45-9) information, including chemical properties, structure, melting point, boiling point, density, formula, molecular weight, uses, prices, suppliers, SDS and more, available at Chemicalbook.

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.

HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid) is a versatile buffering agent essential for biological research, cell culture, and pharmaceutical applications. Its low toxicity and excellent pH stability make it ideal for enhancing experimental accuracy in …