TaDa combines DamID with the GAL4 system in Drosophila, allowing Dam-fusion proteins to be expressed only in specific cell-types at precise temporal windows. TaDa is simple, robust, sensitive and highly reproducible, and works over a wide …

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Dec 22, 2020 · Targeted DamID (TaDa) is an increasingly popular method of generating cell-type-specific DNA-binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new transgenic fly lines for every protein that is profiled, which is both time-consuming and costly.

Jan 18, 2021 · Targeted DamID (TaDa) is an increasingly popular method of generating cell-type-specific DNA-binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new transgenic fly lines for every protein that is profiled, which is both time-consuming and costly.

Targeted DamID (”TaDa”) is based on DNA adenine methlyltransferase identification (DamID). The Escherichia coli DNA adenine methyltransferase (Dam) protein is fused to a DNA- or chromatin-binding protein of interest and the resulting fusion protein methylates binding sites in …

Aug 6, 2020 · Targeted DamID (TaDa) is an increasingly popular method of generating cell-type specific DNA binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new transgenic fly lines for every protein that is profiled, which is both time-consuming and costly.

Targeted DamID (TaDa) is an increasingly popular method of generating cell-type-specific DNA-binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new...

Targeted DamID (TaDa) is an increasingly popular method of generating cell-type specific DNA binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new transgenic fly lines for every protein that is profiled, which is both time-consuming and costly.

Feb 1, 2022 · A separate line is required to profile each DNA-associated protein, and preexisting lines are available for RNA polymerase profiling and chromatin protein profiling. New TaDa lines can be created by cloning and transgenesis using the …

Targeted DamID (TaDa) is an increasingly popular method of generating cell-type-specific DNA-binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new...