3 days ago · The continuous emergence of new SARS-CoV-2 variants requires updating of the S antigen expressed by the B/HPIV3 vector to optimize the immunogenicity of this vector vaccine. While antigenic breadth and cross-protection against different variants following immunization with injectable COVID vaccines has been characterized in many studies, less ...

1 day ago · SARS-CoV- 2 continues to evolve, producing novel Omicron subvariants through recombinant lineages that acquire new mutations, undermining existing antiviral strategies. The viral fitness and adaptive potential of SARS-CoV- 2 present significant challenges to emergency treatments, particularly monoclonal antibodies, which demonstrate reduced efficacy with the emergence of each new variant ...

The cells were fixed with 60% methanol/40% acetone and immunostained with anti-SARS-CoV-2 N mouse monoclonal antibody (ProSci catalog no. 35–579), followed by HRP-conjugated goat anti-mouse IgG (ImmunoReagents Inc., Raleigh, North Carolina, USA), and developed with an AEC Substrate Kit (Vector Laboratories, Newark, California, USA).

Dec 16, 2024 · 15 easy and precise methods for quantification of infection. Here, we developed a SARS-CoV-2 viral 16 vector composed of all four SARS-CoV-2 structural proteins constitutively expressed in 17 lentivirally transduced cells, combined with an RNA replicon deleted for SARS-CoV-2 structural

Aug 5, 2021 · In this review, we describe the biological features of adenoviral (Ad) vectors that position them as potential frontrunners in contrast with other technologies, particularly mRNA vaccines, and...

In this study, we developed a novel SARS-CoV-2 viral vector composed of the 4 SARS-CoV-2 structural proteins (S, E, M, N) carrying a self-amplifying VEEV replicon, a putative SARS-CoV-2 packaging signal, and a reporter gene.

Mar 14, 2025 · Severe acute respiratory syndrome coronavirus (SARS-CoV) (1–4) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (5–9) have crossed the species barrier to infect humans.Both SARS-CoV and SARS-CoV-2 have been identified to use their spike (S) proteins on the virus surface to bind human angiotensin-converting enzyme 2 (hACE2) as the receptor to enter human cells (5, 10).

5 days ago · Our primary dataset comprises 297,186 SARS-CoV- 2 nucleotide sequences, categorized into 3502 distinct lineages. Furthermore, we validated our models using dengue and HIV sequences to demonstrate robustness across different viral datasets. ... and constructing a feature vector by counting the occurrences of each possible k-mer, with larger \(k ...

3 days ago · Author summary In this study, we present a pipeline for designing a high-affinity nanobody (Nb) targeting the SARS-CoV-2 spike protein using enhanced sampling molecular dynamics simulations and CDR-grafting. To address the challenges of CDR grafting in Nbs, including the need for structural similarity between the CDR motif of interest and the scaffold region, we utilized the Nb scaffold ...

Nov 3, 2020 · We identified 14 proteins that were predicted to interact with host RNAs or RNA binding proteins, based on published data for SARS-CoV and SARS-CoV-2. Here, we describe a series of affinity-tagged and codon-optimized expression constructs for each of these 14 proteins.