Apr 22, 2015 · R-250 vs. G-250: Which One Should You Use? Basically, both R-250 (R signifies the slightly reddish tint in the blue color of the dye) and G-250 (G signifies the greenish tint in the dye) have relatively high sensitivity and allow for easy detection (they develop intensely colored complexes upon binding with protein molecules).

Coomassie R-250 and G-250 dyes are two most common chemical forms of Coomassie dyes, as disulfonated triphenylmethane compounds. The R-250 (red-tinted) lacks two methyl groups that are present in the G-250 (green-tinted) form. The "250" originally denoted the purity of the dye.

CBB G-250 is a greenish-blue dye that is more commonly used for staining proteins in polyacrylamide gels, while CBB R-250 is a reddish-blue dye that is often used for staining proteins in agarose gels. Both dyes have similar sensitivity and staining efficiency, but their color differences make them more suitable for specific applications.

考马斯蓝R-250和G-250染料是考马斯染料的两种最常见的化学形式，是二磺化的 三苯基甲烷化合物。 R-250（红色）缺少以G-250（绿色）形式存在的两个甲基。 “250”最初表示染料的纯度。 不同的颜色是染料分子不同带电状态的结果。 当pH值小于0时，考马斯蓝G250染料会腐蚀所有带正电荷的三个氮原子，颜色为红色最大吸收波长为465nm。 当pH值约为1时，染料的总电荷为+1（2-磺酸基团带负电，因为它们的pKa极低），所以染料是绿色的，具有吸收能力最大值 …

G250 is primarily used for bradford. R250 for SDS PAGE (more desirable for protein detection of the two). In addition, G250 is present in 3 states (cationic - red, neutral - green, anionic -...

Brilliant Blue G-250 and Brilliant Blue R-250 are both synthetic dyes commonly used in various applications. However, they differ in terms of their chemical structures and properties. Brilliant Blue G-250 is a triarylmethane dye, while Brilliant Blue R-250 belongs to the xanthene dye family.

Aug 19, 2013 · 考马斯亮蓝G250，又名 Xylene brilliant cyaninG。比考马斯亮蓝R250多二个甲基。MW=854；λmax=590―610nm。染色灵敏度不如R250，但比氨基黑高3倍。优点在于它在三氯乙酸中不溶而成胶体，能选择地染色蛋白而几乎无本底色。

Coomassie Brilliant Blue is available in two forms, R-250 (red-tinted) and G-250 (green-tinted). G-250, also called colloidal coomassie dye, has additional two methyl groups. Both types of Coomassie Brilliant Blue dye reversible stains proteins, therefore, are very useful in …

Coomassie brilliant blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie brilliant blue G-250 differs from Coomassie brilliant blue R-250 by the addition of two methyl groups.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation.