Here, we review the applications, advantages, and challenges of single-molecule fluorescence imaging in plant systems from the perspective of methodology. Firstly, we provide a general overview of single-molecule imaging methods and their principles.

Nov 23, 2023 · MALDI, DESI and secondary ion mass spectrometry (SIMS) are the three most commonly used ion sources for analyzing the small molecular components of plants.

Oct 4, 2024 · Here, we proposed a novel molecular imaging platform that combines sliver nanowire-doped Ti 3 C 2 MXene (Ag NWs@MXene) flexible film substrate with laser desorption/ionization mass spectrometry imaging (AMF-LDI-MSI) to study the spatial distribution of biomolecules on the surface of living plants.

Sep 11, 2021 · Practical sessions included (i) nanosensors and imaging protein–protein interactions at nanoscale, (ii) two-photon deep-tissue imaging and cell ablation, (iii) chemistry-enabled and time-gated imaging, (iv) five-dimensional imaging of development and (v) microfluidic-enabled functional imaging.

Oct 13, 2015 · A resurgence in surface-based analytical technologies and application of molecular imaging techniques is enabling new spatial and temporal exploration of metabolic processes in plant biology.

Jan 13, 2024 · Hyperspectral microscope imaging (HMI), infrared-based microscope imaging (IFMI), and Raman microscope imaging (RMI) combine optical microscopy and spectral technology to nondestructively examine morpho-chemical properties from single cells to …

Jul 11, 2024 · Mass spectrometry imaging (MSI) has become increasingly popular in plant science due to its ability to characterize complex chemical, spatial, and temporal aspects of plant metabolism.

Jul 28, 2022 · This chapter introduces the basic principles of PET, factors that affect the quantitative accuracy of PET when imaging plants, and techniques for administering radiotracers to plants for a variety of molecular plant imaging applications.

Mar 8, 2011 · We present an overview of MALDI-imaging MS (MSI) technology and its use for the analysis of plant tissue. Recent methodological developments have been summarized, and the major challenges involved in using MALDI-MSI, including sample preparation, the analysis of metabolites and peptides, and strategies for data evaluation are all discussed.

Apr 9, 2025 · Other RNA imaging systems that have been used on plant viruses include the stem-loop binders MS2 capsid protein (MS2CP) on turnip crinkle virus with two tags and a 22-amino acid peptide from bacteriophage λ N protein (λN 22) on potato virus A with 16 tags . Neither of these studies observed labelling of vRNA inside PD.