To develop and evaluate a two-dimensional LC system with charged aerosol detection for the simultaneous characterization of protein PEGylation and unreacted PEGylation reagent. The use of polyethylene glycol (PEG) as a safe and low cost additive for the pharmaceutical and cosmetic industries is common practice.

Thermo Scientific Pierce MS (PEG) n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) activated as NHS esters for covalent pegylation of primary amines on proteins (e.g., lysines) or assay surfaces.

HILIC separation can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing the amount of acetonitrile.

The PEG-switch assay is a fast effective semi-quantitative method for measuring target reversible cysteine oxidation in complex protein mixtures derived from tissue or cultured cells.

Jun 1, 1996 · A colorimetric method for quantitative assay of polyethylene glycol (PEG) described here is based on partitioning of a chromophore present in ammonium ferrothiocyanate reagent from an aqueous to a chloroform phase in the presence of PEG.

Using PEG.2 as a positive control antibody, a generic Acoustic Membrane Micro Particle assay using the ViBE instrument platform for the detection of anti-PEG antibodies in human serum has been successfully developed.

This micro-PEG assay is used to evaluate various broadly neutralizing mAb candidates to HIV-1 viral spike (gp120 and gp41 glycoproteins). Using ∼1 mg of VRC01-WT mAb per assay, the precision of the micro-PEG assay was established.

Nov 9, 2021 · Here, we present an automated method based on the titration of a crowding agent (polyethylene glycol, PEG) to quantitatively assess relative solubility of proteins using about 200 µg of...

Analysis of gastrointestinal permeability is based on the appearance of orally administered probes such as sugars, 51 chromium-labelled ethylenediamine tetra-acetic acid (51 CrEDTA), and polyethylene glycol (PEG) in the circulation and urine after permeation of the intestinal epithelium.

Jun 9, 2018 · Procedure for analysis for Polyethylene Glycol in pharmaceutical quality control laboratory. 1. Description. Solid grades occur as practically odorless and tasteless white, …