A Morpholino, also known as a Morpholino oligomer and as a phosphorodiamidate Morpholino oligomer (PMO), is a type of oligomer molecule (colloquially, an oligo) used in molecular biology to modify gene expression.

Apr 20, 2018 · Phosphorodiamidate morpholino oligomers (PMO) are short single-stranded DNA analogs that are built upon a backbone of morpholine rings connected by phosphorodiamidate linkages. As uncharged nucleic acid analogs, PMO bind to complementary sequences of target mRNA by Watson–Crick base pairing to block protein translation through steric blockade.

Jul 15, 2022 · Phosphorodiamidate morpholino oligonucleotides (PMOs) constitute 3 out of the 11 FDA-approved oligonucleotide-based drugs in the last 6 years. PMOs can effectively silence disease-causing genes and modify splicing.

Apr 20, 2018 · Phosphorodiamidate morpholino oligomers (PMO) are short single-stranded DNA analogs that are built upon a backbone of morpholine rings connected by phosphorodiamidate linkages. As uncharged nucleic acid analogs, PMO bind to complementary sequences of target mRNA by Watson-Crick base pairing to block …

We have designed phosphorodiamidate morpholino oligomer (PMO) AOs to various exons of the human dystrophin gene. PMOs were designed to have their target sites overlapping areas of open RNA structure, as defined by hybridization-array analysis, and likely exonic splicing enhancer (ESE)/silencer sites on the target RNA.

Phosphorodiamidate morpholinos (PMOs) are known as premier gene knockdown tools in developmental biology. PMOs are usually 25 nucleo-base-long morpholino subunits with a neutral phosphorodiamidate linkage. PMOs work via a steric blocking mechanism and are stable towards nucleases’ inside cells.

• Modify RNA Processing: PMOs can be used to alter splicing patterns, leading to the production of different protein isoforms. • Probe RNA Structure: PMOs can be used to investigate RNA secondary structures and interactions with other molecules. …

Feb 25, 2022 · Phosphorodiamidate morpholino oligomers (PMOs) are uncharged DNA analogs with therapeutic potential due to their ability to specifically bind to target sites on RNA. By steric inhibition of translation initiation complexes, PMOs can block translation.

Apr 16, 2025 · All PMO-RNA and PMO-PMO Circular Dichroism experiments were performed using 2 μM concentrations of each strand and 0.04 M phosphate buffer in a JASCO J-1500 Spectropolarimeter. Scanning rate 200 ...

Jul 20, 2021 · To rapidly prototype potential PMO drug candidates, we report a fully automated flow-based oligonucleotide synthesizer. Our optimized synthesis platform reduces coupling times by up to...