Nov 28, 2023 · Inspired by these studies, we established the HA-tag-based HOXA9 ChIP-seq using an inducible expression system and identified 229 reproducible HOXA9-bound peaks in MLL-r leukemia cell...

Mar 12, 2009 · Gene expression analyses demonstrate a distinct profile for MLL -rearranged leukemias with consistent high-level expression of select Homeobox genes, including HOXA9. Here, we investigated the effects of HOXA9 suppression in MLL -rearranged and MLL -germline leukemias using RNA interference.

Dysregulation of the oncogenic transcription factor HOXA9 is a prominent feature for most aggressive acute myeloid leukemia cases and a strong indicator of poor prognosis in patients. Leukemia subtypes with hallmark overexpression of HOXA9 include those carrying MLL gene rearrangements, NPM1c mutati …

In mouse cell models, HXR9 affects cell survival of murine hematopoietic progenitor cells transduced with MLL-AF9 or with both HOXA9 and PBX3. Interestingly, HXR9 reduced cell viability in a variety of HOXA7/9/11-positive cell line and blasts from patients but not on HOXA7/9/11-negative ones.

HOXA9 is of particular interest as it has been shown to be over expressed in more than 50% of acute myeloid leukemias and is highly associated with poor prognosis (5–9). A variety of upstream genetic alterations can lead to dysregulation of HOXA9, including MLL-translocations, NUP98-fusions, NPM1c mutations, CDX dysregulation and MOZ-fusions ...

Mar 12, 2009 · Gene expression analyses demonstrate a distinct profile for MLL-rearranged leukemias with consistent high-level expression of select Homeobox genes, including HOXA9. Here, we investigated the effects of HOXA9 suppression in MLL-rearranged and MLL-germline leukemias using RNA interference.

MLL-fusion proteins enforce high-level HOXA9 expression, which is required for transformation in most experimental models (25, 26). This mechanism involves recruitment of at least two complexes important for transcriptional regulation.

Oct 1, 2020 · Aberrant HOXA9 expression is a hallmark of most aggressive acute leukemias, notably those with KMT2A (MLL) gene rearrangements. HOXA9 overexpression not only predicts poor diagnosis and outcome but also plays a critical …

Oct 8, 2018 · We show that HOXA9 functions as a pioneer factor at de novo enhancers and recruits CEBPα and the MLL3/MLL4 complex. Genetic deletion of MLL3/MLL4 blocks histone H3K4 methylation at de novo enhancers and inhibits …

Jun 1, 2015 · HOXA9 is a homeodomain-containing transcription factor that has an important role in hematopoietic stem cell expansion and is commonly deregulated in acute leukemias. A variety of upstream...