Jan 23, 2017 · In order to define a core set of MLL rearranged targets, we investigated the genome-wide binding of the MLL-AF9 and MLL-AF4 fusion proteins and associated epigenetic signatures in acute myeloid...

In patients, MLL-AF4 is predominantly associated with lymphoid malignancies, whereas MLL-AF9 more often results in myeloid malignancies . At the same time, particularly, the lymphoid MLL-r leukemias retain a substantial amount of lineage infidelity and lineage plasticity.

Aug 25, 2023 · In leukemias controlled by the MLL-AF4 fusion protein, we use the ultra-high resolution technique Micro-Capture-C (MCC) to show that MLL-AF4 binding promotes broad, high-density regions of...

Nov 25, 2021 · We use CRISPR-Cas9 gene editing of primary human fetal liver hematopoietic cells to produce a t (4;11)/ MLL-AF4 translocation, which replicates the clinical features of infant-ALL and drives...

Nov 25, 2021 · In this issue of Blood, Malouf et al characterize 2 microRNAs (miRNAs) that are effectors of malignant transformation by the MLL-AF4 fusion protein. 1 Mixed lineage leukemia (MLL) gene fusions are the molecular hallmark of infant acute lymphoblastic leukemia (ALL) and are present in tumor cells in up to 80% of patients. 2 Detection of the gene ...

Jan 1, 2017 · Understanding the underlying molecular mechanisms of defined cancers is crucial for effective personalized therapies. Translocations of the mixed-lineage leukemia (MLL) gene produce fusion proteins such as MLL-AF4 that disrupt epigenetic pathways and cause poor-prognosis leukemias.

Aug 25, 2023 · In leukemias controlled by the MLL-AF4 fusion protein, we use the ultra-high resolution technique Micro-Capture-C (MCC) to show that MLL-AF4 binding promotes broad, high-density regions of enhancer-promoter interactions at a subset of key targets.

In this study, we seek to understand the role of MLL-AF4 in enhancer function as a model to investigate mechanisms of aberrant enhancer activation in cancer.

Nov 14, 2016 · MLL-Af4 induces a B ALL distinct from MLL-AF9 through differential genomic target binding of the fusion proteins leading to specific gene expression patterns. MLL-Af4 cells can assume a myeloid state under environmental pressure but retain lymphoid-lineage potential.

Jan 31, 2012 · Pre-leukemic MLL-AF4 fusions arise prenatally and typically lead to overt acute lymphoblastic leukemia (ALL) at or shortly after birth. In a recent study, Bueno and colleagues explored the...