Sep 13, 2019 · Irisin induced C2C12 cell proliferation and upregulated the mRNA levels of markers of proliferation Pcna, Mki67, and Mcm2. Irisin increased extracellular signal-regulated kinase (ERK) phosphorylation, and U0126, an ERK pathway inhibitor, suppressed irisin-induced C2C12 cell proliferation.

Aug 1, 2021 · To confirm impaired proliferation of myoblasts in the presence of simvastatin, we quantified mRNA expression of Ki-67, a marker of cell proliferation, in myoblasts treated with simvastatin for 24 h and in gastrocnemius of mice treated for 21 days with simvastatin.

Decreased expression of the proliferation factor Ki-67 and of ADAM-12 were also observed in gastrocnemius of mice treated with simvastatin. In conclusion, myoblasts were more susceptible to the toxic effects of simvastatin and simvastatin …

May 1, 2014 · The C2C12 cell line is a subclone from a myoblast line obtained from the thigh muscle of adult C3H mice muscle following crush injury. These cells are capable of rapid differentiation upon serum withdrawal and give rise to contracting myotubes expressing muscle-specific proteins.

The number of Ki-67-positive cells was significantly increased upon coculture with H pylori in comparison with cells cultured alone (Figure 6B). We also performed ELISA with the BrdU assay to confirm the proliferation of C2C12 cells.

Using the C2C12 cell line as a model to study muscle differentiation, we show that calpain 3 is implicated in the early phases of myogenic differentiation, where it participates in the generation of a pool of reserve cells.

Sep 1, 2020 · We found a reduced number of Ki-67-positive cells upon TRAIL knockdown at the onset of differentiation (Fig. 4 G), which was accompanied by an increased number of C2C12 cells expressing the early myogenic marker myogenin (Fig. 4 H).

In the retina, a significant increase in the number of Ki-67-positive cells (marker of proliferating cells) was found in the apical region of the NBL of infected mice compared to the control.

May 14, 2017 · Here we show that cell-cycle regulation underlies variable Ki-67 expression in all situations analyzed, including nontransformed human cells, normal mouse intestinal epithelia and adenomas, human cancer cell lines with or without drug treatments, and …

Jan 23, 2014 · Ki-67 (Santa Cruz, USA) was used as a marker to evaluate cell proliferation. Thirty-six hours after adenovirus treatment, the percentages of Ki-67-positive cells in the Ad-GFP and the Ad-Sirt2 treatment groups were 12.33% and 18.01%, respectively, and the Ki-67 expression was increased by Sirt2 overexpression ( Fig. 2 B ; P < 0.01).