Here we review the role of the major histone PTMs, including phosphorylation, ubiquitination, methylation and acetylation, and their interactions during DNA DSB-induced responses. KEYWORDS: DNA damage, chromatin MODIFICATIONS, Histones.

Here, we review histone methylation in the context of the DDR, focusing on DNA double-strand breaks (DSBs), a particularly toxic lesion that can trigger genome instability and cell death.

In this review, we summarize histone modifications that occur during DNA DSB repair processes. Ionizing radiation (IR) induces many types of DNA damage but the most lethal lesions are DNA double-strand breaks (DSBs), the repair of which is critical for cell survival.

Jun 24, 2014 · Dynamic changes in histone modification are critical for regulating DNA double-strand break (DSB) repair. Activation of the Tip60 acetyltransferase by DSBs requires interaction of Tip60 with histone H3 methylated on lysine 9 (H3K9me3).

Jan 16, 2025 · Here, we review how histone modifications underpin the widespread bidirectional crosstalk between different DNA processes, which allow seemingly distinct phenomena to operate as a unified whole.

Apr 9, 2025 · MDC1 binding then continues the cascade that involves H2AX K13-K15 ubiquitination by the RING-type E3 ubiquitin ligases RNF8 and RNF168 (82–85) and histone H4 K20 methylation (86, 87), ultimately leading to 53BP1 recruitment to DSBs. Our data demonstrate an increase in γH2AX but a decrease in MDC1 and 53BP1, after inducing DSBs selectively ...

2 days ago · Indeed, depletion of the linker histone H1 impairs DSB repair 17,18,19. ... H3K4 methylation promotes the binding of the nucleosomal acetyltransferase complex to acetylate histone H3 at Lys14 ...

Oct 18, 2018 · Double-strand breaks (DSBs) are extremely detrimental DNA lesions that can lead to cancer-driving mutations and translocations. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two main repair pathways operating in the context of chromatin to ensure genome stability.

3 days ago · The Tudor domain of 53BP1 interacts with histone modifications, including H3K9 and H4K20 methylation, which are critical for 53BP1 recruitment to DSBs [].Specifically, SUV39H1 is a major ...

Sep 11, 2024 · Although both histone modifying events are necessary for DSB movement, our results suggest these are two independent pathways. One intriguing possibility is that demethylation and acetylation independently change the biophysical properties of heterochromatin to allow repair.