Both glutamine and glutamate at the N termini of recombinant monoclonal antibodies can cyclize spontaneously to pyroglutamate (pE) in vitro. Glutamate conversion to pyroglutamate occurs …

Pyroglutamic acid (pyroGlu) is commonly observed at the N-terminus of therapeutic monoclonal antibodies. Notably, the term "pyroGlu" refers to a single product that could originate from the …

N-terminal cyclization, also called pyroglutamate formation (pyro-Glu), is one of the major N-terminal modifications of mAbs. proGlu variants are generated by the rearrangement of …

Endoproteinases Asp-N and Glu-C have been used for protein characterization for over 30 years and have gained importance recently due to advancements in mass spectrometry techniques. …

Oct 11, 2006 · The N-terminal glutamic acid (Glu) can be cyclized to form pyroglutamate (pGlu). Recent studies have suggested that N-terminal pGlu formation is an important …

We discovered that the N-term glutamine of QLYSALANK peptide of the endogenous REL2 chain B was nearly completely modified (~98.6%) to pyroglutamic acid (pyro-Glu, qLYSALANK).

Oct 1, 2019 · We have conclusively demonstrated here that the cyclization of N-terminal Glu to pyroGlu increases net molecular charge and produces a basic charge variant with higher pI …

Nov 19, 2019 · Pyroglutamate (pGlu), a cyclization product of N-terminal Gln or Glu residues, is a widespread post-translational modification in proteins, including monoclonal antibodies (mAbs).

Due to its much slower deamidation rate comparing to that of asparagine (Asn), studies on glutamine (Gln) deamidation have been scarce, especially on the differentiation of its isomeric …

Glutamic acid (symbol Glu or E; [4] known as glutamate in its anionic form) is an α-amino acid that is used by almost all living beings in the biosynthesis of proteins. It is a non-essential nutrient …