Feb 1, 2023 · Cap2 methylation specificity is largely guided by mRNA age. We found that this age-directed mechanism for Cap2 methylation provides a novel strategy to distinguish viral RNA from self RNA and...

In this review we explore the regulation of mRNA cap formation and its impact on mammalian cells. The mRNA cap is a highly methylated modification of the 5′ end of RNA pol II-transcribed RNA.

Only recently has the role of the cap 2′O methylation as an identifier of self RNA in the innate immune system against foreign RNA has become clear. The discovery of the cytoplasmic capping machinery suggests a novel level of control network.

Capping of cellular mRNA occurs co-transcriptionally and in vivo requires that the capping apparatus be physically associated with the RNA polymerase II elongation complex. Certain capped mRNAs undergo further methylation to generate distinct cap structures.

Dec 11, 2024 · Here we generate a single-molecule fluorescence imaging system to examine the dynamics with which eIF4F discriminates productive and non-productive locations on full-length, native mRNA...

The mRNA cap is a highly methylated modification of the 5' end of RNA pol II-transcribed RNA. It protects RNA from degradation, recruits complexes involved in RNA processing, export and translation initiation, and marks cellular mRNA as "self" to …

... 5′-ends of eukaryotic cellular mRNAs possess a cap structure, in which an N7-methylguanine (m 7 G) moiety is linked to the first transcribed nucleotide by a 5′-5′ triphosphate bridge...

RNase T2 selectively releases Cap1 and Cap2 from mRNA in the form of two-nucleotide-long and three-nucleotide-long cap tags. c, CapTag-seq. mRNAs are decapped and a biotinylated 5′ end adapter...

Jan 18, 2024 · Among the hundreds of discovered RNA chemical modifications, RNA 5′-end capping with a cap structure is a hallmark of RNA polymerase II (RNAP II)-transcribed RNAs that are essential for RNA processing and transport functions. The textbook cap structure of eukaryotic RNAs is a methylated base, namely m 7 G (N7 -methylguanosine).

1 day ago · Different cytosolic RNA viruses approach this problem in a variety of ways, with some encoding their own RNA-modifying enzymes to introduce the signature mRNA caps [84–88], others employing cap proteins , and some actively recruiting the ribosome to avoid cap recognition entirely . Influenza, on the other hand, employs a very different strategy.