To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

But what is real time RT–PCR? How does it work? How is it different from PCR? And what does this have to do with nuclear technology? Here’s a handy overview of the technique, how it works and a few ...

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

During these thermal cycles, DNA primers bind to the target DNA sequence ... Copying and quantifying DNA at the same time using real-time PCR One modification of conventional PCR allows ...

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

Multiplex real-time PCR-based diagnostic tests facilitate the simultaneous detection of multiple pathogens in a single assay, ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

SCTIMST researchers develop cost-effective, real-time LAMP assay for early TB diagnosis with high sensitivity and specificity.

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...