His method relies on the occurrence of the primer-dimer artifacts in PCR reactions that compete with template DNA. The ratio of input DNA/primer-dimers will determine the amount of template DNA ...

PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka Metropolitan University scientists have developed a light-induced DNA ...

A prerequisite for accuracy is that all possible sources of nonspecific amplification in the PCR reactions are carefully excluded. The formation of primer dimers was eliminated by designing PCR ...

Utilizing the insights gained from single cell, we also present the application of a novel PCR technique, dimer-avoided-multiplex PCR (dam-PCR), to the all-in-one repertoire amplification of BCRs and ...