PCR was developed in 1983 ... For example, if primers bind to each other, the dye will bind to these so-called primer dimers and generate a signal. In addition, SYBR Green I cannot be used ...

View Full Profile. Learn about our Editorial Policies. Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA ...

His method relies on the occurrence of the primer-dimer artifacts in PCR reactions that compete with template DNA. The ratio of input DNA/primer-dimers will determine the amount of template DNA ...