COLD-PCR exploits a small but critical difference in amplicon melting temperature. COLD: Coamplification at lower denaturation temperature; RS: Reference sequence; T c: Critical denuration ...

2). EvaGreen® is also compatible with multiplex PCR, as the lack of dye migration from amplicon to amplicon enables the detection of multiple PCR products by melt curves. This is thanks to EvaGreen® ...

Because uMRT provides full 5'-3' RNA transcript coverage, PCR amplicon location along a first-strand cDNA molecule synthesized by uMRT does not affect qPCR quantification. This greatly reduces the ...

For amplicons you may use the “divide by 50 rule” where you divide the base pair size of the amplicon by 50 to determine the nanograms needed. Template and primer for plasmid DNA 3000 to 5000bp 150ng ...

Overlay PCR reaction with 15 μl of mineral oil to ... This occurs more frequently with larger amplicons (>200 bp). The factors that might be responsible are the amount of time a sample is placed ...