All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the forward primer in a final 50 μl reaction volume (Perkin-Elmer Standard PCR reaction buffer, 0.2 mM dNTPs ...