At the height of every battle taking place, and right when Kurogiri gets freed from the hospital by Spinner, he appears before Toga and asks what she desires. She then takes the vial of Twice's ...

Knowing that Kurogiri was now on their side ... Deku is losing all his quirks against Shigaraki in MHA. I might not have had it for long, but it's taken root in my quirkless self.