The results, published June 22 in eLife, suggest that mutagenesis caused by ENU could be used as an alternative to worms ... However, generating mutations with N-ethyl-N-nitrosourea and then linking ...

They analyzed 878 pedigrees (murine family trees) comprising a total of 45,261 animals and detected 45,000 mutations induced ...

Also, as sequencing power has increased, the need for ultra-high-resolution mapping of ENU-induced mutations has diminished. Our own laboratory uses SSLPs for most mapping applications ...

For instance, many recessive mutations segregate in each G1 animal in subsequent crosses, making it necessary to conduct complicated phenotype analyses. A second limitation to ENU mutagenesis is ...