are post-translationally attached to proteins through a sequential enzymatic cascade that uses an E1 activating enzyme, an E2 conjugating enzyme and an E3 ligase. It was presumed that a single E1 ...

The poly-ubiquitin chain is synthesized by a cascade reaction involving three enzymes, E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin-ligating enzyme ...